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OxyFile #25 


 TI    The effect of ozone (03) on lymphocyte populations in 
       normal and HIV1-infected blood.

 DT    9403

 AU    Wagner K; Mayers D; Toro L; Baker JR Jr; Henry M Jackson 
       Foundation, Rockville, MD., USA

 SO    Int Conf AIDS. 1989 Jun 4-9;5:656 (abstract no. C.587). 
       Unique Identifier : AIDSLINE ICA5/00346989

 AB    OBJECTIVE: Measure the effect of varying 03 concentrations 
       on lymphocytes in whole blood from an uninfected and a 
       Walter Reed Stage 2 HIV1 patient. METHODS: Heparinized 
       whole blood samples were exposed in triplicate to (03) of 
       20, 40, and 60 ug/ml with an oxygen control. Coded, 
       blinded blood samples were gently agitated for 10 min. and
       incubated for 1 hr. at 27 C. Mononuclear cells were 
       separated using Ficoll-hypaque gradients and stained for 
       FACS analysis using labelled monoclonal antibodies. 
       RESULTS: 03 had no effect on lymphocyte populations of the 
       uninfected donor as numbers of total T cells, CD4, CD8, 
       and B cell subpopulations did not change. In contrast, 
       there were marked changes in the lymphocyte populations of 
       the HIV positive donor with increasing (03): TABULAR DATA, 
       SEE ABSTRACT VOLUME. CONCLUSIONS: Ozone, at concentrations 
       previously shown to inactivate HIV1, may alter lymphocyte 
       surface markers in HIV1 infected patients. Further studies
       are indicated to examine this effect.

 DE    Flow Cytometry  Human  HIV Infections/*IMMUNOLOGY  HIV-
       1/*DRUG EFFECTS Leukocyte Count  Lymphocytes/*DRUG EFFECTS  
       Ozone/*PHARMACOLOGY MEETING ABSTRACT

 SOURCE: National Library of Medicine.  NOTICE: This material may 
 be protected by Copyright Law (Title 17, U.S.Code).

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